Thursday, April 29, 2010

information on breeding cannabis, make your own strain!

this guy gives a great breakdown on cannabis breeding, it would be an enormous amount of work, but if one has the passion then i feel it could a joy to do so!

copy and pasted from the grasscity forums, here:
http://forum.grasscity.com/absolute-beginners/387690-what-ibl-strain.html

fadervader breaks it on down:

Breeding and growing cannabis strains is all about manipulating gene frequencies. Most strains sold by reputable breeders through seed banks are very uniform in growth. This means that the breeder has attempted to lock certain genes down so that the genotypes of those traits are homozygous.

Imagine that a breeder has two strains: Master Kush and Silver Haze. The breeder lists a few traits that they particularly like (denoted by *).

This means they want to create a plant that is homozygous for the following traits and call it something like Silver Kush.
Silver Kush
Pale green leaf Hashy smell Silver flowers Short plants

All the genetics needed are contained in the gene pools for Master Kush and Silver Haze.The breeder could simply mix both populations and hope for the best or try to save time, space and money by calculating the genotype for each trait and using the results to create an IBL.

The first thing the breeder must do is to understand the genotype of each trait that will be featured in ideal "Silver Kush" strain. In order to do this the genotype of each parent strain for that same trait must be understood. Since there are four traits that the breeder is trying to isolate, and 4x2 = 8, eight alleles make up the genotypes for these phenotype expressions and must be made known to the breeder.

Let's take the pale green leaf of the Silver Haze for starters. The breeder will grow out as many Silver Haze plants as possible, noting if any plants in the population display other leaf colors. If they do not, the breeder can assume that the trait is either homozygous dominant (SS) or recessive (ss). If other leaf colors appear within the population, the breeder must assume that the trait is heterozygous (Ss) and must be locked down through selective breeding. Let's look closely at the parents for a moment.

If both parents were SS there wouldn't be any variation in the population for this trait. It would already be locked-down and would always breed true without any variations.

With one SS parent and one Ss parent, the breeder would produce a 50:50 population — one group being homozygous (SS) and the other heterozygous (Ss).

If both parents were Ss, the breeder would have 25 percent SS, 50 percent Ss and 25 percent ss. Even though gene frequencies can be predicted, the breeder will not know with certainty whether the pale green leaf trait is dominant or recessive until they perform a test cross. By running several test crosses the breeder can isolate the plant that is either SS or ss and eliminate any Ss from the group. Once the genotype has been isolated and the population reduced to contain only plants with the same genotype, the breeding program can begin in earnest. Remember that the success of any cannabis growing and breeding program hinges on the breeder maintaining accurate records about parent plants and their descendants so that they can control gene frequencies.

Let's say that you run a seed bank company called PALE GREEN LEAF ONLY BUT EVERYTHING ELSE IS NOT UNIFORM LTD. The seeds that you create will all breed pale green leaves and the customer will be happy. In reality, customers want the exact same plant that won the cannabis cup last year or at least something very close. So in reality, you will have to isolate all the ^winning' traits before customers will be satisfied with what they're buying.

The number of tests it takes to know any given genotype isn't certain. You may have to use a wide selection of plants to achieve the goal, but nevertheless it is still achievable. The next step in a breeding program is to lock down other traits in that same population. Here is the hard part.

When you are working on locking down a trait you must not eliminate other desirable traits from the population. It is also possible to accidentally lock down an unwanted trait or eliminate desired traits if you are not careful. If this happens then you'll have to work harder to explore genotypes through multiple cross tests and lock down the desired traits. Eventually, through careful selection and record keeping you'll end up with a plant that breeds true for all of the features that you want. In essence, you will have your own genetic map of your cannabis plants.

Successful breeders don't try to map everything at once. Instead, they concentrate on the main phenotypes that will make their plant unique and of a high quality. Once they have locked down four or five traits they can move on. True breeding strains are created slowly, in stages. Well known true breeding strains like Skunk#l and Afghani#l took as long as 20 years to develop. If anyone states that they developed a true breeding strain in one or two years you can be sure that the genetics they started with were true breeding, homozygous, in the first place.

Eventually you will have your Silver Kush strain but only with the four genotypes that you wanted to keep. You may still have a variety of non-uniform plants in the group. Some may have purple stems, while others may have green stems. Some may be very potent and others not so potent. By constantly selecting for desired traits you could theoretically manipulate the strain into a true breeding strain for every phenotype. However, it is extremely unlikely that anyone will ever create a 100 percent true breeding strain for every single phenotype. Such a strain would be called a perfect IBL. If you're able to lock down 90 percent of the plant's phenotypes in a population then you can claim that your plant is an IBL.

The core idea behind the true breeding technique is to find what is known as a donor plant. A donor plant is one that contains a true breeding trait (homozygous, preferably dominant for that trait). The more locked down traits are homozygous dominant the better your chances of developing an IBL, which does not mean that the line of genetics will be true breeding for every trait, but rather that the strain is very uniform in growth for a high percentage of phenotypes.

Wednesday, April 28, 2010

extreme foxtailing business, weird sativa phenotypes

i have an interest in unique plants, i have seen several that display a very odd extreme foxtail phenotype and i am very much curious about these

i will start off by bringing up quaze, sometimes referred to as quasar, barney's farm has seeds for sale that are being called "dr. grinspoon" after the late dr., rumors state that barney's refers to "dr. grinspoon" as "quaze 2.0", quaze himself states that dr. grinspoon is in fact not his quaze strain?! so who bloody knows! for $55.-- american one can order it and find out here:
http://www.cannabis-seeds-bank.co.uk/barneys-farm-dr-grinspoon/prod_1360.html

here's the attitude seed shop description:
Sex : Feminized
Type : Sativa
Flowering : Photoperiod Genetics : Heirloom Sativa strain
Flowering Time : Long Outdoor Harvest : Mid November
Height : Tall THC Level : 25%
Characteristics : Grows tall and requires longer flowering time
Barneys Farm Dr Grinspoon is named in honor of our hero, the brilliant cannabis advocate, researcher and author Dr. Lester Grinspoon. It's genetics is a pure heirloom Sativa. The plant grows thin and tall, and needs a longer flowering time around 95-100 days. When mature, the colas look like compact, green and red-brown beads, loosely strung on thin stems with a dusting of THC crystals. The fragrance off the buds is of sumac and lemon and the smoke is light and tangy with citrus and tropical fruit aromas. Dr Grinspoon can stretch very tall indoors. You can harvest these cannabis seeds outdoors in Mid November. Dr. Grinspoon has a very high THC of 25% so it offers a strong, long-lasting, energetic and cerebral high. Old school Sativa of the highest order.

here are some pictures of quaze's quaze strain, apparently this is being sold in various shops in amsterdam and commanding a high price, from 15 - 17 pounds/gram



here is what quaze has to say about growing it out, this is copypasta'd from icmag mostly:
http://www.icmag.com/ic/showpost.php?p=2161134&postcount=17
Indoor
let her grow for 10 days then flower about depends how many seeds you want that this girly makes for you we did 14 to 15 and the seeds where ripe

after 8 to 9 weeks she makes this little yellow bananas inside the calyx
http://www.icmag.com/ic/showpost.php?p=2157461&postcount=1
Look at this nice Candysticks almost no leafs and full of resin .

yes its a beauty and its a female landrace sativa don`t ask me where its coming from because we grow this candy out of a seed .
and god nows for how long its being hiding .

she polinates herself but most landraces do the same its normal to servive in nature if there is no male around.

she is covered with resin sticky like honey the taste is amazing, smell like fresh lemon can`t explain you have to smoke yourself.
shortly after quaze made the thread on icmag, esbe chimed in with pics of his mighty mite that displays a very similar characteristic:
http://www.icmag.com/ic/showpost.php?p=2157508&postcount=3
lovely! looks a bit like the mighty mite from breederbrad i had od last season





quaze had this to say about esbe's mighty mite grow:
http://www.icmag.com/ic/showpost.php?p=2158054&postcount=8
looks a little little bit like the Quasar ESBE but its not the same .
the quasar makes a lot of calyxes maybe far away familie
titoon29 mentions the f13 phenotype that displays the same characteristics:
http://www.icmag.com/ic/showpost.php?p=2161623&postcount=21
i remember an f13 from dj on IC which does big calyxes like this, with this incredible foxtail style^^ but i can t find the picts... maybe someone remember this f13 ?

gentlefarmer says the same thing but brings us some pictures of this dj short f13 extreme foxtail phenotype, he thinks it may be related to highland thai genetics in dj's f13, things that make ya' go hmmmm:
http://www.icmag.com/ic/showpost.php?p=2178720&postcount=26
I had a very unique girl from a bag of DJ Shorts F-13 (the pics above) the F-13 was/is a prodject to pull some of DJ's Highland Thai's genes to the surface of his strains. just a thought when i saw your VERY nice girl. Maybe she desends from southeast asia just my two cents,

Grow for all the right reasons, Peace. GF





quaze has this to say about gentlefarmer's f13:
http://www.icmag.com/ic/showpost.php?p=2180462&postcount=27
GentlemanFarmer that is a realy nice thai you have DJ short F-13 yep beautifull looking

and look like the Quaze wauw amazing I appreciate what you have found here

the buds are more dens then on the Quaze and also the big fan leafs are in the bud o

wauw love this strain you have there already is this Thai making seeds also ????

thank you very much for your reply`s and effort you put in to this
lastly we have some comments by l33t, he grew out some la nina from mr nice seedbank which also shows extreme foxtailing, here's what he says and a couple pictures:
http://www.icmag.com/ic/showpost.php?p=2183019&postcount=35
the Quaze buds looks bit similar to one of my La Nina (MrNiceSeedbank) as I mentioned over the Revival of the Ultimate Sativa thread

I had also culled a LN plant that looked very similar to your mighty mite

It had a very strong intoxicating unique smell , perhaps I should have let it finish but at the time had other better (more practical) plants that I decided to focus on



last but not least, here are some words from another user, indifferent, on the subject of quaze:
http://www.icmag.com/ic/showpost.php?p=3449141&postcount=31
I was gifted a load of the Quaze last year, it's okay, but to me, it smokes just like a typical highland Mexican, and I suspect it is just a freak pheno of a highland Mexican. The taste is similar to the Oaxacan I have as is the high, so the reason i don't rate it is there are tons of plants with the same level of taste and high but which do grow proper buds and yield good. Most highland Mexicans yield big, so i just don't get the appeal of this Quaze, especially because it is a hermie and it's male flowers ain't sterile.

Truly, honestly, Quaze just shows how long it is since proper sativas were available in Amsterdam, so people have forgotten what they are like. There is nothing at all extraordinary about the Quaze high, it's good but so are a lot of proper sativas.

Saturday, April 24, 2010

baby grow cab update - april 24 2010

i was gone for a few days, i thought they'd be okay with the water i gave them right before i left, well i was wrong, we lost a few of the babies, this is what's left


also here is a group shot again, we are going to switch to flowering tonight, i just don't want the thai getting too big like the xj13 did last time, want to give it plenty of room height wise

trim some for clones! - april 22 2010

we made cuts on the thai, kandy kush x skunk, and jack the ripper in order to get clones!

you can see that they are all a bit more bare now, we'll switch to flowering mode very very soon here

Wednesday, April 21, 2010

how do you track your plants? - april 21 2010

while perusing the various growers forums, i saw a thread about plant tracking software, there were 2 programs mentioned that could be used to help someone keep track of their plants and at what stage they are in

i thought it was a super cool idea, so i decided to put together a little spreadsheet that would help me with this, i'm calling it plantrackit, this little tool has been helping me a lot and since it's just in excel it is super quick and easy to customize, here is a screenshot


the idea is that the user types into the green portion, the calculations take care of themselves everywhere else

this is 0.2, the change was the estimated finish time column (est finish (weeks)), the idea here is that your plants can still be vegging, you can plug in an estimated finish time, then type in a start date for flowering, your stop date is now calculated based on the estimated finish time, i don't think this is done very cleanly so i'll probably redo it for 0.2.1 or something, i feel like it should give an estimated stop date as well as a separate real stop date, hmmm...maybe a progress bar too, can excel do that? hmmm i'm sure there's a way, so flowering will have a progress bar from 0 - 100% depending on how close we are to the estimated finish time

a column for preflowering might be nice also, currently i am just appending an - F or an - M if it's male or female (actually, if it's male, i yank it and delete that plant from the spreadsheet), a preflower column along with another date column for the preflowering

now that i think about it and am rambling, it might be a good idea to add a completely separate schedule somehow for feedings as well, would nested spreadsheets for each plant be the best way? if we're going to delve that deep then i feel excel may no longer be the best option, might be best to just go ahead and write a real program for it, which i would really prefer not to get into

either way, i use this, it helps, i'll keep on updating it as needed, this is a nice way to help keep organized

Monday, April 19, 2010

cheese had nanners so it got chopped, popped in the backup cheese - april 19 2010

so the cheese was indeed a male, after careful examination all over the node sites with a magnifying glass, there is no mistake that sticks and balls were visible all over the place, i got rid of it and transplanted the second cheese plant i had going, we should know very soon if it's a male or not, this is the game we play with non feminized bag seeds =)

here it is pulled out of the ground and about to go into the trash bag, this is the first plant i have ever intentionally killed, sad to do it, later dude


here's the new one going in, you can see the roots growing out of the soil clump that was at the bottom of the plastic beer cup it was living in...this plant is the same age as the male, it didn't get nearly as large though i'm guessing because it was in a beer cup which is so small, hope this one's a girl!



lastly here is a group shot, in the beer cup to the left in the back is a strawberry cough from bag seed, an actual strawberry plant next to that, in front to the left is the new cheese i transplanted, in the middle is jack the ripper, the right front is kandy kush x skunk, and the back right is thai

preflowers? i think we got some nanners a hangin' - april 19 2010

2 of the plants appear to have preflowers, the one below appears to be female, the second one looks like a male at this point, debating on chopping or waiting a few more days on the male looking one


Sunday, April 18, 2010

update - april 18 2010

not much to say here, plants are growing, we went away camping for a couple days and came back, they had definitely grown and gained a couple nodes

Thursday, April 15, 2010

budget led installed, transplant and strawberries - april 15 2010

we'll start off with all the bigger girls vegging, in order of appearance, thai, jack the ripper, kandy kush x skunk, bagseed cheese





the led arrived in the mail today, i installed it into the baby sprout/vegetating area so now we wait and see what happens after a few days, pardon the dark picture as i'm still working out some things with this camera


















a friend gave me some strawberry plants, they are kicking it under the light for a short while; i also transplanted 2 ganja girls into slightly larger containers, we're just using your standard disposable beer cup for the time being

Wednesday, April 14, 2010

new sprout and the thai - april 14 2010

i planted a random bag seed a few days back, well today it sprouted, who knows what it could be!

also here is a pic of the thai plant, giving it some kelp based nutrients for N seemed to have helped, it's looking greener and isn't getting any more of the spots, none of the other plants are showing the spots either, hooray kelp!

Tuesday, April 13, 2010

cheap-o ebay led panels - april 13 2010

i had money sitting in paypal for who knows how long, only like $50, so i decided to play around and get a cheapo quad band led panel to help supplement the cfls in the baby chamber, the led panel is only 13 watts, i am not expecting any massive results at all but i am curious if the panel will actually help my baby plants any

this is the same model that i had purchased, mine was $34

here's some info copied from the amazon ad:

Product Features

  • Solid state, Cooler running, High efficiency, Wide angle, Quad Band full spectrum plant lighting
  • 96 Red light LEDs: 640nm, 60 Blue light LEDs: 465nm, 56 Orange Light LED: 620 n 24 White Light LED: 8000K
  • Recommended coverage: one panel per sq. ft
  • Power: 13.8 Watts, Input Voltage 120 Volt

baby area is back in effect, added some mylar to main area - april 13 2010

i need to finish mylaring this grow closet, you can see that the plants are looking better after i gave them some kelp based nutrients to help with N deficiency, since i have the babies out of here i lowered the lamp a bit, i should probably move the 2 small plants still in their biodegradable pot into something larger, thinking some plastic beer cups for now


i've set up an area for sprouts and babies again, we have 3 cfls in there right now and will be adding a small cheap led panel in about a week


Friday, April 9, 2010

thai grows fast! - april 9 2010

wow it's almost as big as the tallest ones planted 2 weeks prior, i am going to transplant a few of these in just a moment, beginning to show sex already and i don't see any nads so we should be good to go, may try to fit a 600watt hps in as well if i have time tonight

Wednesday, April 7, 2010

4 new sprouts! - april 7 2010

i woke up and looked and saw 3 more sprouts, 2 white rhino and 1 3rd dimension, came back from work and had a 3rd white rhino! here's some pics, i'll post a couple pics of the other plants as well



Monday, April 5, 2010

more round 2 shots - april 5 2010

here are a few shots of round 2 and the progress there

you can see a few of the girls got burned, i gave them nutrients far too early, strawberry cough and la woman showed signs first and are not doing too well on the recovery front, the thai plant looks like it was hardly phased, jack the ripper as well, cheese recovered super quick, kandy kush x skunk looked like it wasn't going to make it but has a fair amount of nice green new growth